FIG 7.

The cyclic peptomer 4EpDN inhibits secretion of the effector protein ExoU but not the regulator ExsE. PA103 was grown under T3S-inducing conditions in the presence of 9 μM or 60 μM cyclic peptomers or DMSO. (A) Secretion of ExoU was visualized using Coomassie blue and quantified. (B) In the same samples, Western blotting was carried out for secreted ExsE in the supernatant and ExsE in the cell pellets. BSA and RpoA were used as loading controls. Data were from three independent experiments. One-way ANOVA with Dunnett’s multiple-comparison test was used. *, P < 0.05; ****, P < 0.0001 compared to DMSO. Error bars are standard errors of the mean.