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. 2021 Jun 17;65(7):e00289-21. doi: 10.1128/AAC.00289-21

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Copyright © 2021 Janice et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Genomic integrations into the sRNA PrrF1/PrrF2 loci of P. aeruginosa. (A) DNA sequence encoding the two regulatory small RNAs (PrrF1 and PrrF2; bold) with promoter elements (−35 and −10) and DRs (159/156 bp; boxed in gray) indicated. Red triangles mark insertion sites of GIs. (B) Alignments of GIs (n = 7) detected in the indicated strains, where the chromosomal insertion site is represented by strain 1334/14. Open reading frames are indicated by arrows pointing in the direction of transcription, with genes belonging to the chromosomal backbone (blue), genomic islands (orange), resistance (red), insertion sequence (IS) element (yellow), and the xerD (turquoise) or int (green) recombinase/integrase-encoding genes highlighted. PAGI-97B (light yellow), In1595 (green), and DRs (magenta) are boxed, and a gray scale for DNA identity (67 to 100%) is shown.