FIGURE 6.

Surviving young mice show increased number of CD45^loP2RY12^neg myeloid cells. 16 week and 85 week mice were infected with 100 PFU WNV‐NY via footpad inoculation. At 30 dpi, mice that survived the infection were euthanized, and leukocytes were isolated from the cortices and analyzed by flow cytometry. (a) Representative flow cytometry plots of CD11b⁺‐gated cells analyzed by CD45 vs P2RY12 expression with gating for P2RY12^neg cells. (b) Quantification of number of P2RY12^negCD11b⁺ cells in each group. (c) Representative flow cytometry plots of P2RY12^neg‐gated cells analyzed by P2RY12 vs CD68 expression. (d) Quantification of number of CD68⁺P2RY12^neg cells in each group. (e) Representative flow cytometry plots of P2RY12^neg‐gated cells analyzed by P2RY12 vs MHCII expression. (f) Quantification of number of MHCII⁺P2RY12^neg cells in each group. These data are the representative of two experiment with 7–15 mice per group. Statistical significance was calculated using one‐way ANOVA with Welch's correction and Dunnett's multiple comparisons test. Exact p values noted; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001