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. 2021 Aug 14;14:3959–3967. doi: 10.2147/JIR.S322247

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© 2021 Haddad.

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Images depicting L6 skeletal muscle myoblasts from rats, differentiated into L6 skeletal muscle myotubes (passage 7). (A) Myoblasts obtained on day 3 of the tissue culture in 10% FBS Ham F-10 media (10X). (B) Cells obtained on day 4 of the tissue culture in 6% horse serum Ham F-10 media (10X). (C) Myotubes obtained on day 5 of the tissue culture in 2% horse serum Ham F-10 media (10X). (D) Myotubes obtained after 4 hours of tissue culture in 2% delipidated serum Ham F-10 media (10X). (E) Myotubes obtained after one hour of cell starvation in only Ham F-10 media (10X). (F) Myotubes obtained after 19 hours of cell starvation in only Ham F-10 media (10X), (scale bar 50μm).