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. 2021 Aug 14;14:3959–3967. doi: 10.2147/JIR.S322247

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© 2021 Haddad.

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L6 muscle myotubes—ACEA, Rimonabant, ACEA and Rimonabant, and insulin affect IL-6 gene expression; myotubes fed with delipidated serum. The stimulation time covered 1 to 24 hours, and IL-6 mRNA levels, relative to TATA-Box, was evaluated by quantitative real-time PCR (qRT-PCR) (100 nM). The following scenarios explain the stimulation process conducted: (A) stimulation was done for 1 hour. (B) Stimulation was done for 3 hours. (C) Stimulation was applied for up to 5 hours. (D) Stimulation was applied for up to 24 hours. The data were reported as the mean ± SEM of three separate groups. (n=3; *denotes p=0.05 and **denotes p=0.01). Data were investigated by conducting one-way ANOVA test and Tukey’s test.

Abbreviation: IL-6, Interleukin 6.