FIGURE 5.

A high‐fat diet increased nuclear 8‐oxoguanine accumulation in granule cells and reduced the volume of the granule cell layer in the hippocampus of App^{NL−F/NL−F} mice. (a) Representative images of the Nissl‐stained hippocampus of App^{NL−F/NL−F} and wild‐type mice fed an RD or HFD. Scale bar = 200 µm. (b) GCL volume estimation using the Cavalieri method with 5 serial sections per brain showed that the volume of GCL was decreased in App^{NL−F/NL−F} mice fed HFD. (c) Representative images of the immunohistochemical detection of 8‐oxoG in nuclear DNA in the hippocampus of App^{NL−F/NL−F} and wild‐type mice fed an RD or HFD. Scale bar = 200 µm. (d) The quantitative measurement of the 8‐oxoG intensity in nuclear DNA in 3 zones of hippocampus: GCL, MSS, and CA1sp. Data expressed as the mean ± SEM, n = 4, and four brain slices were examined per mouse. The results were statistically analyzed by a two‐way ANOVA (p values for each analysis shown) followed by post hoc Tukey's HSD test, ^* p < 0.05, and ^** p < 0.01