TABLE 1.
Cell type | Marker gene | Relative expression (% RD‐fed wild‐type mice) | ||
---|---|---|---|---|
HFD‐fed wild‐type | RD‐fed AppNL−F/NL−F | HFD‐fed AppNL−F/NL−F | ||
Astrocyte | Aqp4 | 96.42 | 120.13 | 107.82 |
Gfap | 102.86 | 115.10 | 128.01 | |
Glul | 99.98 | 107.68 | 108.20 | |
S100b | 94.57 | 110.69 | 107.27 | |
Slc1a1 | 99.27 | 104.44 | 104.10 | |
Slc1a2 | 98.19 | 109.86 | 99.00 | |
Mean | 98.55 | 111.32 | 109.07 | |
SD | 2.88 | 5.57 | 9.90 | |
Oligodendrocyte | Mag | 112.78 | 103.41 | 124.33 |
Mbp | 107.55 | 108.34 | 111.51 | |
Mog | 110.97 | 98.34 | 115.80 | |
Sox10 | 111.30 | 102.11 | 109.95 | |
Mean | 110.65 | 103.05 | 115.40 | |
SD | 2.21 | 4.13 | 6.45 | |
Microglia | Aif1 | 100.60 | 98.21 | 94.12 |
C1qa | 121.59 | 130.97 | 139.62 | |
C1qb | 116.39 | 120.24 | 132.32 | |
Cd68 | 128.68 | 145.24 | 165.39 | |
Itgam | 98.89 | 96.71 | 104.91 | |
Lgals3 | 102.29 | 98.30 | 108.48 | |
Ptprc | 117.32 | 108.44 | 145.07 | |
Mean | 112.25 | 114.02 | 127.13 | |
SD | 11.64 | 18.83 | 25.49 | |
NSC/NPC | Ascl1 | 99.32 | 112.66 | 110.23 |
Bmp4 | 111.59 | 127.28 | 160.18 | |
Dcx | 105.75 | 119.54 | 96.37 | |
Egf | 94.50 | 101.41 | 104.82 | |
Igf2 | 150.47 | 148.18 | 156.45 | |
Notch2 | 123.84 | 113.32 | 99.78 | |
Nr2e1 | 101.36 | 95.07 | 94.97 | |
Shh | 110.79 | 104.09 | 109.80 | |
Sox2 | 98.13 | 106.47 | 94.87 | |
Mean | 110.64 | 114.22 | 114.16 | |
SD | 17.38 | 16.00 | 25.72 | |
Neuron | Chga | 95.33 | 101.57 | 103.23 |
Eno2 | 92.32 | 92.81 | 93.68 | |
Nefh | 88.86 | 103.59 | 91.82 | |
Nefl | 92.69 | 100.71 | 100.92 | |
Nefm | 88.67 | 110.27 | 99.87 | |
Rbfox3 | 94.75 | 90.48 | 93.11 | |
Snap25 | 94.34 | 102.92 | 100.44 | |
Syp | 99.25 | 96.42 | 100.41 | |
Syt1 | 96.74 | 99.58 | 97.54 | |
Tubb2a | 92.63 | 86.51 | 94.41 | |
Mean | 93.56 | 98.49 | 97.54 | |
SD | 2.95 | 8.61 | 2.69 |
Significantly altered genes vs. RD‐fed wild‐type mice (eBayes ANOVA: p < 0.05) are underlined.
Abbreviations: HFD, high‐fat diet; NPC, neural progenitor cell; NSC, neural stem cell; RD, regular diet.