FIGURE 2.

Alpha‐amylase in hiPSC‐derived neurons and oligomer amyloid beta‐42 stimulated SHSY5Y cells. Image in (a) shows hiPSC‐derived neurons without mutation in PSEN1 gene (isogenic control) (L150P‐GC) immunoflourescently stained against salivary α‐amylase (green) with DAPI marker nuclei (magenta). A hiPSC neuronal process is shown in higher magnification in (b) where the arrow indicates α‐amylase (green) and phalloidin (magenta) in close association. Graph in (c) shows the significant lower levels of α‐amylase in L150P compared to L150P‐GC. Image in (e) shows control (ctrl) SH‐SY5Y cells immunofluorescent staining of α‐amylase (green), and nuclei are visualized with DAPI (magenta). The graph in (f) shows significantly lower α‐amylase concentrations in amyloid beta 42 oligomers Aβ42O stimulated cells compared to Ctrl. Scale bar in (a and e) indicates 15 μm, and scale bar in (b) indicates 0.5 μm. Statistical analysis was done with t test, and values in graphs (c and f) are presented as mean ± SD. ***indicates p‐value < 0.001, **indicates p‐value < 0.0, *p‐value < 0.05