Skip to main content
. 2021 Jul 18;20(8):e13430. doi: 10.1111/acel.13430

FIGURE 7.

FIGURE 7

Forkhead Box O1 directly regulates SMS1 transcription. (a and b) DPSCs at 12P were transfected with LV‐ROR2, si‐FOXO1 vector, or their control vectors, and SMS1 level was measured by (A) RT‐qPCR and (B) Western blot analysis. (c) Three putative SMS1 binding elements within the 2‐kb promoter region of FOXO1 were identified. (d) ChIP‐qPCR was used to determine FOXO1‐SMS1 binding to the SMS1 promoter region in DPSCs (12P); **p < 0.01 compared to IgG. (e) T cells (n = 293) were transfected with the indicated constructs; luciferase activity was measured by dual‐luciferase reporter assays. (f) DPSCs at 12P were transfected with LV‐ROR2, si‐FOXO1 vector, or their control vectors, and the level of SM was measured. (g and h) DPSCs at 12P were transfected with si‐FOXO1, pcDNA3.1‐SMS1 vector, or their control vectors, and SA β‐gal staining was performed. DPSCs at passages 3–12 used in these experiments were from young donors. For all analyses, *p < 0.05, **p < 0.01, and ***p < 0.001 compared to their corresponding controls, unless specified