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. 2021 Aug 4;12:663482. doi: 10.3389/fphys.2021.663482

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Copyright © 2021 Lin, Wang, Zhao, Wu, Su, Zhang, Shen, Wu, Qian and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of expression patterns of BmCPV-miRNAs and target gene BmRan in the midgut of silkworm larvae infected with BmCPV. (A) BmCPV-miR-1 and BmCPV-miR-3 detected by stem-loop RT-PCR. The bands sized about 70–80 bps were the putative miRNAs. c: control; t: BmCPV infected; t1: BmCPV-miR-1; t2: BmCPV-miR-3. (B) Expression pattern of BmCPV-miR-1. (C) Expression pattern of BmCPV-miR-3. (D) Expression pattern of BmRan with normalization to different internal reference genes. The expression of BmCPV-miRNAs gradually increased, while the expression of the target gene BmRan gradually decreased, as the infection advanced (n = 3 replicate samples, each containing five larvae).