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. 2021 Aug 4;12:663482. doi: 10.3389/fphys.2021.663482

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Copyright © 2021 Lin, Wang, Zhao, Wu, Su, Zhang, Shen, Wu, Qian and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Regulation of BmCPV-miRNAs on the target gene in HEK293T cells and in BmN cells. (A) BmCPV-miRNAs were expressed in the transfected HEK293T cells. (B) BmCPV-miRNAs downregulate the expression of the substituent target gene mCherry in HEK293T cells. pLNHX-Ran: pLNHX-mCherry-RanUTR; pLNHX-Ran-mut: pLNHX-mCherry-RanUTR-mut. (C) Regulation of BmCPV-miRNA mimics on expression of target gene BmRan in BmN cells (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001).