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. 2021 Aug 4;12:663482. doi: 10.3389/fphys.2021.663482

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Copyright © 2021 Lin, Wang, Zhao, Wu, Su, Zhang, Shen, Wu, Qian and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Regulation of miRNA mimics and inhibitors on replication of the BmCPV genome in midgut of silkworm larvae. Result shows that the BmCPV-miRNAs could enhance the replication of BmCPV genomic RNAs. (A1) BmCPV-miR-1 to S2; (B1) BmCPV-miR-3 to S2; (C1) BmCPV-miR-1 + BmCPV-miR-3 to S2; (A2) BmCPV-miR-1 to S5; (B2) BmCPV-miR-3 to S5; (C2) BmCPV-miR-1 + BmCPV-miR-3 to S5; (A3) BmCPV-miR-1 to S10; (B3) BmCPV-miR-3 to S10; (C3) BmCPV-miR-1 + BmCPV-miR-3 to S10. S2, S5, and S10 represent the 2nd, 5th, and 10th segment of BmCPV genomic RNA, respectively (n = 3 replicate samples, each containing five larvae, *p < 0.05, **p < 0.01, ***p < 0.001).