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. 2021 Aug 4;12:663482. doi: 10.3389/fphys.2021.663482

TABLE 1.

Primer sequences for qRT-PCR.

Primer name Sequence (5′–3′)
Stem-loop reverse-transcribed PCR for BmCPV-miR-1
RT: GTCGTATCCAGTGCAGGGTCCGAGGT
ATTCGCACTGGATACGACTAGTGT
F: ACACTCCAGCTGGGGAAATGGACACAGGC
Stem-loop reverse-transcribed PCR for BmCPV-miR-3
RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCA
CTGGATACGACATCAAGCC
F: ACACTCCAGCTGGGTAGGAGAATTAGCGCGG
Universal R: CCAGTGCAGGGTCCGAGGTA
mCherry F: CTCAGTTCATGTACGGCTCCAAGG
R: GGAGTCCTGGGTCACGGTCAC
Human β-actin F: CTCCATCCTGGCCTCGCTGT
R: GCTGTCACCTTCACCGTTCC
BmRan F: GCCGTAACGACTTTGCTTTGGAAC
R: TTGCCAGTACCACCATCTCCTACC
Genomic RNA S2 F: GTTGAGCGTCAGCAGTCAGATCG
R: TGTTTACCCTGAGCAGCGTTATCG
Genomic RNA S5 F: CGCTTACAGGCAGTGGAATAGGAC
R: GCTCTAACACATCGCTGGGCTAAG
Genomic RNA S10 F: ACCGTCAGTGATTGCTCGTGTAAC
R: AGCGTCACCCTATCCGAAGACC
Bmβ-actin F: CCGTATGAGAAAGGAAATCA
R: TTGGAAGGTAGAGAGGGAGG