TABLE 1.
Primer name | Sequence (5′–3′) |
Stem-loop reverse-transcribed PCR for BmCPV-miR-1 | |
RT: GTCGTATCCAGTGCAGGGTCCGAGGT ATTCGCACTGGATACGACTAGTGT |
|
F: ACACTCCAGCTGGGGAAATGGACACAGGC | |
Stem-loop reverse-transcribed PCR for BmCPV-miR-3 | |
RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCA CTGGATACGACATCAAGCC |
|
F: ACACTCCAGCTGGGTAGGAGAATTAGCGCGG | |
Universal | R: CCAGTGCAGGGTCCGAGGTA |
mCherry | F: CTCAGTTCATGTACGGCTCCAAGG |
R: GGAGTCCTGGGTCACGGTCAC | |
Human β-actin | F: CTCCATCCTGGCCTCGCTGT |
R: GCTGTCACCTTCACCGTTCC | |
BmRan | F: GCCGTAACGACTTTGCTTTGGAAC |
R: TTGCCAGTACCACCATCTCCTACC | |
Genomic RNA S2 | F: GTTGAGCGTCAGCAGTCAGATCG |
R: TGTTTACCCTGAGCAGCGTTATCG | |
Genomic RNA S5 | F: CGCTTACAGGCAGTGGAATAGGAC |
R: GCTCTAACACATCGCTGGGCTAAG | |
Genomic RNA S10 | F: ACCGTCAGTGATTGCTCGTGTAAC |
R: AGCGTCACCCTATCCGAAGACC | |
Bmβ-actin | F: CCGTATGAGAAAGGAAATCA |
R: TTGGAAGGTAGAGAGGGAGG |