Table 2.
Minimum inhibitory concentrations (MICs), phenotypic test for β-lactamases, β-lactamase content and plasmid incompatibility group of the tested isolates
| No | Strain number/protocol number | Specimen | Category | Age and gender | TZP | CZ | CXM | CAZ | CTX | CRO | FEP | IMI | MEM | GM | CIP | COL | ESBL | HODGE | CIM | BL content | PFGE cluster Plasmid Inc group |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | KC-4559 | Urine (catheter) | XDR | 46 M | >128 | >128 | >128 | >128 | >128 | >128 | 64 | 16 | 32 | 32 | 64 | 16 | + | - | + | IS1999OXA-48 CTX-M(1) | 1, L |
| 2 | KC-5887 | Stool | MDR | 74F | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 0.5 | 2 | 16 | 64 | 8 | + | - | - | CTX-M(1) | 2, NEG |
| 3 | KC-5874 | BAL | MDR | 80 F | >128 | >128 | >128 | >128 | >128 | >128 | 16 | 0.5 | 0.5 | 64 | >128 | 16 | + | - | - | IS1999OXA-48 CTX-M | 1, L |
| 4 | KC-5852 | Urine (catheter) | XDR | 41 F | >128 | >128 | >128 | 32 | >128 | >128 | 16 | 4 | 4 | 16 | >128 | 16 | + | + | - | IS1999OXA-48 CTX-M | 2, NEG |
| 5 | KC-7985 | Urine | XDR | M | >128 | >128 | >128 | >128 | >128 | >128 | 64 | 32 | 64 | >128 | 32 | 16 | - | + | + | NDM-1 | L/M |
| 6 | KC-5831 | BAL | MDR | 80 M | >128 | >128 | >128 | >128 | >128 | >128 | 32 | 2 | 2 | >128 | >128 | 64 | + | + | + | IS1999OXA-48 CTX-M | 1, L |
| 7 | KC-7950 | Urine (catheter) | MDR | 77 M | >128 | >128 | >128 | >128 | >128 | >128 | 16 | 1 | 0.5 | >128 | >128 | 16 | + | + | + | IS1999OXA-48 ISEcpCTX-M | 2.L |
| 8 | KC-6730 | Stool | XDR | 74F | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 8 | 16 | >128 | >128 | 8 | + | + | + | OXA-48 CTX-M, TEM | 4, L, FIIs |
| 9 | KC-6396 | Urine (catheter) | XDR | 46 M | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 32 | 32 | >128 | >128 | 8 | + | + | + | IS1999OXA-48 ISEcpCTX-M | 1, NEG |
| 10 | KC-7822 | Urine (catheter) | XDR | 41 F | >128 | >128 | >128 | 32 | >128 | >128 | 16 | 4 | 4 | >128 | >128 | 16 | + | + | + | OXA-48 CTX-M | NEG |
| 11 | KC-10844 | Urine (catheter) | MDR | 48 M | >128 | >128 | >128 | >128 | >128 | >128 | 64 | 8 | 0.5 | >128 | >128 | 32 | + | + | + | OXA-48 CTX-M | 1, NEG |
| 12 | KC-9393 | Urine (catheter) | MDR | 64 F | >128 | >128 | >128 | >128 | >128 | >128 | 64 | 8 | 2 | 64 | >128 | 8 | + | + | - | IS1999OXA-48 CTX-M | 1, NEG |
| 13 | KC-10877 | Urine (catheter) | MDR | 80 M | >128 | >128 | >128 | >128 | >128 | >128 | 8 | 0.06 | 0.06 | 2 | 1 | 16 | + | - | - | ISEcpCTX-M, TEM | S, FIIs |
| 14 | KC-9644 | Urine | MDR | 73 M | >128 | >128 | >128 | >128 | >128 | >128 | 64 | 1 | 0.5 | 64 | >128 | + | - | + | OXA-48 CTX-M | 2, FIIs | |
| 15 | VG2803 | Blood culture | MDR | 81 F | >128 | >128 | >128 | >128 | >128 | >128 | 128 | 0.5 | 1 | 64 | >128 | 32 | + | - | + | OXA-48 CTX-M | 5, FIIs |
| 16 | VG-3203 | Wound swab | MDR | 52 F | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 1 | 2 | >128 | >128 | 32 | + | + | + | OXA-48 CTX-M | 2, FIIs |
| 17 | KC-11559 | Urine (catheter) | MDR | 84 F | >128 | >128 | >128 | >128 | >128 | >128 | 32 | 2 | 2 | 1 | >128 | 32 | + | + | + | IS1999OXA-48 CTX-M | 3, FIIs |
| 18 | KC-11366 | Urine | MDR | 57 M | >128 | >128 | >128 | 32 | >128 | >128 | 16 | 1 | 2 | >128 | >128 | 64 | + | + | + | OXA-48 CTX-M | 2, FIIs |
| 19 | KC-8260 | Tracheal aspirate | MDR | 75 M | >128 | >128 | >128 | >128 | >128 | >128 | 64 | 0.5 | 0.5 | 16 | 128 | 32 | + | - | - | IS1999OXA-48 CTX-M | L, FIIs |
| 20 | G-4229/18 | Urine | MDR | 85 F | >128 | >128 | >128 | 16 | >128 | >128 | 16 | 2 | 2 | >128 | >128 | >128 | + | + | - | OXA-48 CTX-M | 3, NEG |
| 21 | G-33494 | Urine | MDR | 83 M | >128 | >128 | >128 | 32 | >128 | >128 | 8 | 1 | 0.5 | >128 | 0.5 | 32 | + | + | + | OXA-48 CTX-M | 5, NEG |
| 22 | R-37876 | Tracheal aspirate | MDR | 51 M | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 2 | 4 | 16 | >128 | 16 | + | + | + | IS1999OXA-48 ISEcpCTX-M, TEM | 4, L |
| 23 | R-32246 | Tracheal aspirate | XDR | 73 M | >128 | >128 | >128 | >128 | >128 | >128 | 32 | 8 | 16 | 32 | >128 | 16 | + | + | + | OXA-48 CTX-M, TEM | NEG |
| 24 | R-34003 | Tracheal aspirate | XDR | 64 M | >128 | >128 | >128 | 64 | >128 | >128 | 64 | 4 | 32 | 64 | >128 | 16 | + | - | + | IS1999OXA-48 CTX-M, TEM | 4, FIIs |
| 25 | R-48638 | Tracheal aspirate | XDR | 65 M | >128 | >128 | >128 | 64 | >128 | >128 | 16 | 32 | 32 | >128 | >128 | 32 | + | + | + | OXA-48 CTX-M | 4, NEG |
| 26 | KC-14064 | Urine (catheter) | MDR | 82 F | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 2 | 2 | 64 | 32 | 32 | + | - | + | OXA-48 CTX-M | NEG |
| 27 | KC-13630 | Rectal swab | MDR | 81 F | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 4 | 2 | >128 | >128 | 8 | + | - | + | OXA-48 CTX-M | 2, NEG |
| 28 | KC-13635 | Rectal swab | XDR | 89 F | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 64 | 32 | >128 | >128 | 16 | + | - | + | OXA-48 CTX-M | NEG |
| 29 | KC-13933 | BAL | MDR | 74 M | >128 | >128 | >128 | >128 | >128 | >128 | 32 | 2 | 0.5 | 32 | >128 | 16 | + | - | + | OXA-48 CTX-M | 2, FIIs |
| 30 | KC-12390 | Urine (catheter) | MDR | 85 F | >128 | >128 | >128 | >128 | >128 | >128 | 32 | 2 | 0.5 | 32 | >128 | 16 | + | - | + | OXA-48 CTX-M | 2, FIIs |
| 31 | KC-13184 | Rectal swab | XDR | - F | >128 | >128 | >128 | >128 | >128 | >128 | 32 | 64 | 32 | 64 | >128 | 16 | + | - | + | OXA-48 CTX-M | 2, NEG |
| 32 | G-5376 | Urine | MDR | 69 M | >128 | >128 | >128 | 64 | >128 | 64 | 8 | 2 | 4 | 1 | 4 | 8 | + | + | + | OXA-48 CTX-M | NEG |
| 33 | SI-33057 | Urine | XDR | 80 M | >128 | >128 | >128 | >128 | >128 | >128 | 64 | 64 | 64 | 128 | >128 | 32 | + | + | + | OXA-48 CTX-M | 2, NEG |
| 34 | SI-31419 | Urine | XDR | 62 M | >128 | >128 | >128 | >128 | >128 | >128 | 128 | 4 | 32 | 128 | >128 | 16 | + | + | + | OXA-48 CTX-M, TEM | S, NEG |
| 35 | SI-32324 | Intraoperative sample | XDR | 75 F | >128 | >128 | >128 | >128 | >128 | >128 | 32 | 64 | 64 | 1 | >128 | 8 | + | + | + | OXA-48 CTX-M, TEM | 2, NEG |
| 36 | G-14029 | Wound swab | XDR | 84 M | >128 | >128 | >128 | >128 | >128 | >128 | 128 | 8 | >128 | >128 | >128 | 16 | + | + | + | OXA-48 CTX-M, TEM | FIIs |
| 37 | UG17233 | Urine | MDR | 55 M | >128 | 32 | 32 | 0.5 | 1 | 0.5 | 0.25 | 16 | 4 | 0.5 | 2 | 16 | - | + | + | OXA-48 | 2, NEG |
| 38 | UG15750 | Urine | MDR | 83 F | >128 | 64 | 8 | 0.5 | 0.25 | 1 | 0.25 | 16 | 32 | 0.5 | 4 | 16 | - | + | + | OXA-48 | 2, NEG |
| 39 | UG16530 | Urine (catheter) | XDR | 85 F | >128 | >128 | >128 | >128 | >128 | >128 | 16 | 16 | 32 | 64 | 64 | 16 | + | + | + | OXA-48 CTX-M | 2, NEG |
| 40 | G-39684 | Urine | MDR | 76 F | >128 | >128 | >128 | >128 | >128 | >128 | 32 | 1 | 4 | 32 | >128 | 8 | + | + | + | OXA-48 CTX-M, TEM | NEG |
| 41 | G-6643/2-17 | Urine | MDR | 53 M | >128 | >128 | >128 | >128 | >128 | >128 | 64 | 2 | 2 | >128 | 2 | 32 | + | - | + | OXA-48 CTX-M | NEG |
| 42 | SI-4-20 | Blood culture | MDR | 47 M | >128 | >128 | >128 | >128 | >128 | >128 | 64 | 0.5 | 2 | 1 | >128 | 32 | + | + | + | OXA-48 CTX-M, TEM | NEG |
| 43 | DU-2721 | Urine | XDR | 71 M | >128 | >128 | >128 | >128 | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 16 | - | + | + | KPC, TEM | 6, NEG |
| 44 | DU-4076 | Urine | XDR | 19 F | >128 | >128 | >128 | >128 | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 16 | - | + | + | KPC, TEM | NEG |
| 45 | UR3304 | Urine | XDR | 83 F | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 32 | >128 | >128 | >128 | 4 | - | + | + | KPC, TEM | FIIs |
| 46 | DU-5321 | Urine | XDR | 53 M | >128 | >128 | >128 | >128 | >128 | >128 | >128 | 32 | >128 | >128 | >128 | 8 | - | + | + | KPC, TEM | 6, NEG |
ESBL – combined disk test using cephalosporins alone and combined with clavulanate for detection of ESBLs; CIM – carbapenem inactivation method; TZP – piperacillin/tazobactam; CZ – cefazoline; CXM – cefuroxime; CAZ – ceftazidime; CTX – cefotaxime; CRO – ceftriaxone; FEP – cefepime; IMI – I imipenem; MEM – meropenem; GM – gentamicin; CIP – ciprofloxacin; COL – colistin; BL – β-lactamase; MDR – multidrug-resistant; XDR – extensively-drug resistant; BAL – bronchoalveolar lavage fluid; KC – Karlovac hospital; VG – Sestre Milosrdnice Hospital Zagreb; SI – Sisak Public Health Institute; G – Andrija Štampar Public Health Institute Zagreb; DU – Dubrovnik Public Health Institute; UR – urine specimen (Split University Hospital Centre); UG – urogenital specimen (Split University Hospital Centre); RI – Rijeka University Hospital Centre.