Skip to main content
. 2021 Aug 19;54(10):2399–2416.e6. doi: 10.1016/j.immuni.2021.08.016

Figure 5.

Figure 5

Neutralization of SARS-CoV-2 variants by anti-SARS-CoV-2 mAbs

(A) SARS-CoV-2 variants and their mutations in spike.

(B and C) SARS2-2 (B) and SARS2-38 (C) were tested for neutralization of the indicated variants by FRNT. Mean EC50 values are shown; data are from 2–10 experiments. See Figures S4B and S4C for representative dose-response curves.

(D and E) K18-hACE2 mice were administered 100 μg (~5 mg/kg) of the indicated mAb by intraperitoneal injection 24 h prior to intranasal inoculation with 103 FFU of SARS-CoV-2 Wash-B.1.351.

(D) Mean weight change is shown. Error bars represent SEM.

(E) At 6 dpi, the indicated tissues were collected, and viral RNA levels were determined. Data are from two experiments; WEEV-204 (isotype control) and SARS2-38: n = 7; SARS2-02: n = 6.

(F and G) K18-hACE2 mice were inoculated with 103 FFU of SARS-CoV-2 Wash-B.1.351 and 24 h later were administered 200 μg (~10 mg/kg) of the indicated mAb.

(F) Mean weight change. Error bars represent SEM.

(G) At 6 dpi, tissues were collected, and viral RNA levels were determined. Data are from two experiments; hWNV-E16 and hSARS2-02: n = 6; hSARS2-38: n = 8.

(H and I) K18-hACE2 mice were inoculated with 5 × 103 FFU of SARS-CoV-2 B.1.617.1 and 24 h later were administered 200 μg (~10 mg/kg) of the indicated mAb.

(H) Mean weight change. Error bars represent SEM.

(I) At 7 dpi, tissues were collected, and viral RNA levels were determined. Data are from three experiments; hWNV-E16: n = 9; hSARS2-38: n = 10.

(J and K) K18-hACE2 mice were inoculated with 103 FFU of SARS-CoV-2 Wash-B.1.351 and 48 h later were administered 200 μg (~10 mg/kg) of the indicated mAb.

(J) Mean weight change. Error bars represent SEM.

(K) Survival with log-rank test.

In (D) and (F), we used one-way ANOVA with Dunnett’s post-test of the area under the curve. In (H) and (I), we used a t test of the area under the curve. In (E) and (G), we used a Kruskal-Wallis test with Dunn’s post-test. In (I), we used a Mann-Whitney test. For all statistical tests: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.