REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
SARS2-01	This paper	N/A
SARS2-02	This paper	N/A
SARS2-03	This paper	N/A
SARS2-10	This paper	N/A
SARS2-16	This paper	N/A
SARS2-31	This paper	N/A
SARS2-34	This paper	N/A
SARS2-38	This paper	N/A
SARS2-55	This paper	N/A
SARS2-71	This paper	N/A
COV2-2130	Zost et al., 2020	N/A
COV2-2196	Zost et al., 2020	N/A
CR3022	ter Meulen et al., 2006	N/A
hSARS2-02 (chimeric human mAb)	This paper	N/A
hSARS2-38 (chimeric human mAb)	This paper	N/A
Anti-mouse IgG-HRP	Sigma	Cat # A8924, RRID: AB_258426
goat anti-mouse IgA/G/M	SeraCare	Cat# 5210-0187
Bacterial and virus strains
VSV-SARS-CoV-2 (WT and panel of escape mutants)	Liu et al., 2021 and this paper	N/A
SARS-CoV-2 WA1/2020	CDC	N/A
SARS-CoV-2 D614G	Chen et al., 2021	N/A
SARS-CoV-2 Wash-B.1.351	Chen et al., 2021	N/A
SARS-CoV-2 Wash-B.1.1.28	Chen et al., 2021	N/A
SARS-CoV-2 B.1.1.7	Chen et al., 2021	N/A
SARS-CoV-2 B.1.429	C. Chiu and R. Andino labs (UCSF)	N/A
SARS-CoV-2 B.1.1.298	BEI Resources	NR-53953
SARS-CoV-2 B.1.222	BEI Resources	NR-53945
SARS-CoV-2 B.1.617.1	M. Suthar laboratory (Emory University)	N/A
SARS-CoV-2 B.1.617.2	R. Webby laboratory (St Jude Children Research Hospital)	N/A
SARS-CoV-2 B.1.526 (S477N)	M. Suthar laboratory (Emory University)	N/A
SARS-CoV-2 B.1.526 (E484K)	M. Suthar laboratory (Emory University)	N/A
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 spike protein	Fremont laboratory	N/A
SARS-CoV-2 spike RBD	Fremont laboratory	N/A
Deposited data
SARS2-38/RBD structure (local)	This paper	PDB: 7MKM
SARS2-38/RBD EM density map (local)	This paper	EMD-23899
SARS2-38/spike structure (three down conformation)	This paper	PDB: 7MKL
SARS2-38/spike EM density map (three down conformation)	This paper	EMD-23898SARS
SARS2-02 VH sequence	This paper	GenBank: MZ703121
SARS2-02 VK sequence	This paper	GenBank: MZ703122
SARS2-38 VH sequence	This paper	GenBank: MZ703123
SARS2-38 VK sequence	This paper	GenBank: MZ703124
Experimental models: Cell lines
Vero-E6	ATCC	CRL-1586; RRID:CVCL_0574
Vero+TMPRSS2	Chen et al., 2021	N/A
Vero+TMPRSS2+ACE2	Chen et al., 2021	N/A
MA104	ATCC	CRL-2378.1
Calu-3	ATCC	HTB-55
Experimental models: Organisms/strains
K18-hACE2 transgenic mice	Jackson laboratory	strain # 34860
Golden Syrian Hamsters	Charles River	Strain 049
Oligonucleotides
SARS-CoV-2 qPCR primer 5′- ATGCTGCAATCGTGCTACAA-3′	Case et al., 2020	N/A
SARS-CoV-2 qPCR primer 5′- GACTGCCGCCTCTGCTC-3′	Case et al., 2020	N/A
SARS-CoV-2 qPCR probe 5′-/56-FAM/ TCAAGGAAC/Zen/ AACATTGCCAA/3IABkFQ-3′	Case et al., 2020	N/A
GAPDH qPCR primer+probe	IDT PrimeTime Assay	Hs.PT.39a.22214836
Software and algorithms
MotionCor2 v1.3.1	Zheng et al., 2017	N/A
GCTF v1.18	Zhang, 2016	N/A
CrYOLO v1.7.6	Wagner et al., 2019	N/A
Relion 3.1	Scheres, 2012; Zivanov et al., 2018	N/A
cryoSPARC v3.1.0	Punjani et al., 2017	N/A
DeepEMhancer	Sanchez-Garcia et al., 2021	N/A
Coot v0.9.5	Emsley et al., 2010	N/A
Isolde v1.1.0	Croll, 2018	N/A
Phenix v1.19	Adams et al., 2010	N/A
qtPISA	Krissinel and Henrick, 2007	N/A
MDTraj	McGibbon et al., 2015	N/A
UCSF ChimeraX	Goddard et al., 2018	N/A