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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Int J Parasitol. 2021 Mar 25;51(9):761–775. doi: 10.1016/j.ijpara.2021.01.003

Fig. 5.

Fig. 5.

Expression pattern of P34 (Cp-P34) and gp900 (Cp-gp900) of Cryptosporidium parvum during intracellular development in vitro. Fluorescent micrographs (600x) demonstrating Madin-Darby bovine kidney (MDBK) cells with intracellular stages of C. parvum over the course of infection- at 2, 6, 24 and 48 h p.i. Parasites are stained green with FITC-labeled Vicia villosa lectin (VVL); binding of the anti-Cp-P34 VHH dimer (JJS-A6/JJS-A11) and anti-Cp-gp900 VHH dimer (JMP-F7/JMP-F7) are detected in red with TRITC-labeled secondary antibody and the cell nucleus is counter-stained in blue with DAPI. A non-specific VHH dimer is included as a negative control. Scale bar = 5 μm.