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. Author manuscript; available in PMC: 2022 Feb 15.
Published in final edited form as: Cancer Res. 2021 May 27;81(16):4194–4204. doi: 10.1158/0008-5472.CAN-20-3199

Figure 1: LKB1 loss leads to global demethylation in lung adenocarcinoma patients.

Figure 1:

β-values obtained from TCGA LUAD level 1 data. LKB1 status was determined using 16-gene classifier. (A) Average β-values from TCGA LUAD samples with differing LKB1 and KRAS status were compared using Student’s t-test to identify significant changes in average methylation based on status. LKB1-deficient tumors display significant hypomethylation compared to LKB1-WT tumors regardless of KRAS status. (B) Average β-values from CCLE NSCLC cell lines with differing LKB1 and KRAS status were compared using Student’s t-test to identify significant changes in average methylation based on status. LKB1-deficient cell lines display significant hypomethylation compared to LKB1-WT cell lines regardless of KRAS status. (C) Clustering and heatmap visualization was performed on the top 5000 differentially methylated probes between LKB1-loss and LKB1-WT groups. Samples were ranked by LKB1-loss signature score.