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. Author manuscript; available in PMC: 2022 Feb 15.
Published in final edited form as: Cancer Res. 2021 May 27;81(16):4194–4204. doi: 10.1158/0008-5472.CAN-20-3199

Figure 2: Depletion of SAM-e cycle metabolites in LKB1-loss and demethylation of repetitive elements.

Figure 2:

33 resected lung adenocarcinoma samples were characterized for LKB1 status using a NanoString signature and analyzed using LC-MS/MS. (A) Untargeted LC-MS/MS of LKB1-loss patient samples indicated a significant enrichment for alterations of metabolites in the cysteine-methionine metabolic pathway; six pathway metabolites with a statistically significant decrease (p < 0.05) are shown in the pathway map with LogFC. (B) Log2 fold change and Benjamini-Hochberg adjusted p-values were calculated for SAM-e and SAH using the Limma package. (C) The ratio of SAM-e to SAH was determined using the spectral instensities from 2B, Student’s t-test showed a statistically significant decrease in the SAM-e/SAH ratio.