C57/BL6 wild type (WT) mice were treated with STZ to induce diabetes. Cardiac function was assessed with echocardiography 16 weeks after induction of diabetes. (A) Plasma glucose levels were measured 16 weeks after vehicle or STZ treatment. (B) Fractional shortening (FS), (C) E’/A’, (D) e/E’ ratio, and (E) isovolumic relaxation time (IVRT) were measured to evaluate systolic and diastolic functions. (F) Representative pulsed-wave (upper panels) and tissue (middle panels) doppler images and corresponding electrocardiogram (lower panels) images. (G) Cardiac NAD+/NADH ratio were measured. N=6. *: P<0.05 to vehicle. (H) FS, (I) E’/A’ ratio, (J) e/E’ ratio, (K) IVRT and (L) cardiac NAD+/NADH ratio were measured in mice 2-week after diabetic stress. N=6. *: P<0.05 to vehicle.