Sustenance of PKM2pS37 supports tumor growth in vivo. A) Overview of MDA-MB-231 xenograft model generation and experimental workflow. B) Tumor volume measurement for two weeks of treatment with Dinaciclib (50 mg/kg) or TEPP-46 (40 mg/kg). Error bars, SEM; one-way ANOVA (Tukey’s HSD), *P = 0.03, ***P = 0.0002. t test, comparison of vehicle group to treatment groups; ns, nonsignificant. C) Immunoblot analysis and D) immunohistochemical analysis of dissected tumors at treatment end point (day 20 post-implantation). Bars, mean values, n = 3; error bars, SD; one-way ANOVA (Tukey’s HSD), *P = 0.03, **P = 0.002; T, tumor. H&E, Hematoxylin and Eosin staining. Scale bar, 100 μm.