Figure 2.

Aged C3H/He mice fed the ALIOS diet develop HCC. Livers of ALIOS fed mice at 48 weeks appeared steatotic with visible tumours (A), with elevated tumour burden (B) relative to control. Tumour size increased in association with liver/weight ratio (C) and fasting blood glucose (D). The transcriptome of mouse tumours overlapped with a distinct human subclass of HCC, comparing RNA-seq data (E–F). In the heat map of Non-negative Matrix Factorization (NMF) (E),tissue type is colour coded (top row). Red and blue represent higher and lower gene expression. The human non-tumour (NT) metagene is marked V6 (blue rectangle), with tumour (T) metagenes defined as V1–V5 and V7. A red rectangle marks the V3 human metagene. Mouse T and NT transcriptomes are shown by orange and green rectangles. A proliferative subclass was identified by the Gene set enrichment analysis (GSEA) score of top enriched dataset in human V3 (F), and the mouse T versus NT differentially expressed genes (G). Data in (B) presented as mean ± S.E.M., compared with Mann Whitney test.