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. 2021 Aug 18;11:16767. doi: 10.1038/s41598-021-96184-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of secreted proteins that stabilise/destabilise Treg markers. (A) Schematic describing the concept of the Treg assay where FOXP3 and CTLA4 protein expression was used as an indicator of Treg stability. (B) Effect of proteins screened on FOXP3 and CTLA4 expression in Tregs at top concentration tested. The effect was measured as FOXP3 and CTLA4 Median Fluorescence Intensity (MFI) of live cells and normalized to Robust Z Score based on the on-plate PBS neutral controls. The blue dashed lines indicate the threshold for identifying hits based on ≥ 3 × Robust Z-score, or ≤ -3 × Robust Z-score of FOXP3 or CTLA4 expression.