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. 2021 Aug 18;11:16767. doi: 10.1038/s41598-021-96184-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Suggested model of IFNα-7 vs IFNα-10 strengthens difference in ISG transcription signaling. (A) Schematic representation of HEK293 reporter cell line for IFNAR1/2 mediated JAK STAT1/2 signaling leading to ISG transcription of secreted embryonic alkaline phosphatase. (B) Dose–response curves of HEK293 reporter cell line upon treatment with either IFNα-7 (blue circles) or IFNα-10 (green squares), SEM error bars based on n = 2, duplicate measurements and subtracted baseline signal (PBS). (C) A pathway map showing signaling pathways for the IFNAR1/IFNAR2 receptor and the IL-2 receptor. Genes in red are upregulated when comparing IFNα7-treated versus IFNα10-treated cells. JAK3 is significantly upregulated (FDR < 0.05 and > twofold upregulation). * indicates additional phenotypic readouts at a protein level in the primary assay. The observed differences between IFNα-7 and IFNα-10 are further discussed in the main text.