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. 2021 Aug 18;6:48. doi: 10.1038/s41536-021-00159-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — hASCs were cultured on MBP-FGF2 surface, or treated with soluble form of FGF2, or MBP-FGF2 proteins. Total RNAs, proteins, and conditioned medium were prepared and analyzed by RT-qPCR, western blot, and ELISA, respectively. A Effect of various FGF2 ligands on IL-8 RNA. Values were normalized to GAPDH. ns not significant, ***p < 0.001, ****p < 0.0001 (one-way ANOVA), n = 3 per group. B Effect on IL-8 protein. ***p < 0.001, ****p < 0.0001 (one-way ANOVA), n = 3 per group. C Effect on JNK phosphorylation. D Effect on p65 phosphorylation. For western blot analysis, β-actin was used as a loading control. All data are presented as mean ± SD.