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. 2021 Aug 18;6:48. doi: 10.1038/s41536-021-00159-7

Fig. 9. Roles of IL-8 in the tissue regenerative potential of FECS-Ad in mouse hindlimb ischemia.

Fig. 9

Ischemic thigh and TA muscles from PBS, FECS-Ad, or IL-8-silenced FECS-Ad-treated mice were prepared followed by immunofluorescence. A Representative TAs at appropriate times after ischemic surgery are shown in the photos. n = 4 per group. Scale bar, 2 mm. B Time kinetics of TA weights of each group 1, 7, 14, and 28 days after surgery. ns not significant among PBS, FECS-Ad, and FECS-Ad (IL-8 KD) group, *p < 0.0001 compared to PBS group, #p < 0.0001 compared to FECS-Ad (IL-8 KD) group (two-way ANOVA). n = 4 per group. Laminin staining of C ischemic thigh and D TA of each group at 1, 7, 14, and 28 days post-surgery. Nuclei were stained with DAPI. Scale bar, 20 μm. n = 5 per group. Quantification of muscle fibers with at least one centralized nuclei in E ischemic thigh and F TA. ns not significant among PBS, FECS-Ad, and FECS-Ad (IL-8 KD) group, *p < 0.0001 compared to PBS group, #p < 0.0001 compared to FECS-Ad (IL-8 KD) group (two-way ANOVA). n = 5 per group. All data are presented as mean ± SEM.