Table 2.
Characterization of twelve microsatellite markers developed in Gongolaria barbata (15 sites sampled, N = 375 unique genotypes).
| Locus (multiplex) | GenBank accession no | Primer sequences 5′–3′ and ABI fluorescent dyes | Repeat type | Size | Na | HS | FIS |
|---|---|---|---|---|---|---|---|
| Cysbar1 (1) | KT344783 | F:6FAM-ATACAGTAATGAATGGTACAGCG | (TG)12 | 169–204 | 15 | 0.508 | 0.042 |
| R:ACATTCTGCTACGGGGACAG | |||||||
| Cysbar2 (1) | KT344784 | F:6FAM-AGATCCTCTGAAAGGCGGTC | (TG)13 | 87–130 | 10 | 0.242 | 0.099 |
| R:ACTCTGCGACGTTCCTGTAG | |||||||
| Cysbar3 (1) | KT344785 | F:ROX-GCAGGAGTCCGGTGCTAC | (AC)21 | 141–212 | 18 | 0.415 | 0.501*** |
| R:CTATCACGGGTTGGGTTCAC | |||||||
| Cysbar4 (2) | KT344786 | F:HEX-TAGTTGACTGTTTTGCCGCC | (GTT)10 | 75–149 | 23 | 0.439 | 0.216*** |
| R:ACCGTACAAGAGTGTTGCTG | |||||||
| Cysbar5 (1) | KT344787 | F:HEX-AATCAGAAGACGGCGAATGC | (CA)12 | 134–205 | 39 | 0.736 | 0.255*** |
| R:GCGTACCGACTCTTTAGGTG | |||||||
| Cysbar6 (2) | KT344788 | F:6FAM-ACGAAGCCTCTCTATTTTGCC | (CAG)7 | 83–102 | 9 | 0.585 | − 0.710*** |
| R:CCGACTAAAAGCATCGTCCC | |||||||
| Cysbar7 (2) | KT344789 | F:ROX-AGGGGTGCTACAGATGATGC | (CA)14 | 132–165 | 16 | 0.538 | 0.069 |
| R:TCTCAGAAATCCAGCCCGTG | |||||||
| Cysbar8 (2) | KT344790 | F:6FAM-CGTATGGTAGGCATGCTTGG | (TG)13 | 142–172 | 16 | 0.593 | − 0.015 |
| R:CGTATAACACCTGCCTTTAGGAAC | |||||||
| Cysbar9 (2) | KT344791 | F:DRAGONFLY-CGACGTAGCATCGTGTTGTC | (TG)15 | 168–193 | 10 | 0.329 | 0.307** |
| R:GCTAGAACGGTTTGGTGCTG | |||||||
| Cysbar10 (1) | KT344792 | F:6FAM-GTATGGCTCTACTCCCACCC | (AGT)10 | 234–254 | 10 | 0.549 | 0.123*** |
| R:GCATTTTTGCATTCTACCGGC | |||||||
| Cysbar11 (1) | KT344793 | F: DRAGONFLY-AGGCACCCTGGATACTGTTG | (GTA)7 | 240–257 | 5 | 0.449 | 0.220*** |
| R: ATCGCAGTTGATTTCCGCAG | |||||||
| Cysbar12 (2) | KT344794 | F:6FAM-TGCTTTACTGAAACCCCGTC | (CCA)8 | 215–243 | 11 | 0.326 | − 0.003 |
| R:TCGATCTATGTCGTCAGGGC |
Na: Number of alleles, HS: Nei’s genetic diversity within subsample; FIS: fixation index and its p-value from U test for heterozygote deficiency or excess (**p-value < 0.01, ***p-value < 0.001, non-significant for all the other loci). The three loci bolded were removed from the final dataset.