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. 2021 Aug 18;4:981. doi: 10.1038/s42003-021-02512-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic diagram of full length Stx7-SEP, Stx7-SEP lacking the N-terminal domain (Stx7-ΔN-SEP), and Stx7-SEP lacking SNARE motif (Stx7-ΔSNARE-SEP). b Distribution of Stx7-SEP, Stx7-ΔN-SEP, and Stx7-Δ-SNARE-SEP in transfected neurons. Box-whisker plots showing the surface fraction of Stx7-SEP and truncated mutants (c) and vesicular pHs of vesicles carrying respective SEPs (d). Note that vesicular pH of Stx7-ΔSNARE-SEP could not be calculated, since it was exclusively expressed at the cell surface (N.D. indicates ‘not determined’). e Responses of Stx7-SEP (black traces) and Stx7-ΔN-SEP (red traces) upon 10 Hz (left panels) and 40 Hz stimulation (right panels). f Responses of Stx7-ΔN-SEP upon 10-Hz stimulation in control (black) and after TeNT treatment (red). g Responses of Stx7-ΔN-SEP upon 40 Hz stimulation in control (black) and after Lat-A treatment (red).