Figure 6.

MGF-AuNPs produced apoptosis in PC-3 cells and inhibits endothelial cell tube formation: (A,B) PC-3 cells were cultured in 6 well plates for overnight followed by treatment with MGF-AuNPs for 24 h. The cells were harvested and permeabilized. After permeabilization the cells were stained with Annexin V for membrane damage and with Propidium Iodide (PI) for DNA damage. The cells were washed and analyzed by either flow cytometry or fluorescent microscopy; (C) 6 well plates were layered with Matrigel and HAECs were cultured on the Matrigel for tube formation. AuNPs at 41 and 83 µM for 24 h were added to the culture. Tube formation was observed under the light microscope and pictures were taken.