TABLE 3.
Genotypic characteristics of the MRSE isolates.
| Isolate | Resistome (plasmid/chromosomal-mediated) | Plasmid replicon type | R-M system | *SCCmec type | ACME type | MLST | Insertion sequences | No. of CRISPR-Cas elements | Pathogenicity score (no. of pathogenic families) |
| C35 | mecA, blaZ, norA, dfrG, tet(K), cat(pC221), erm(C) | rep15, rep19b, rep19c, rep24c, rep39, rep21, repUS22, rep7a, repUS43, rep10 | – | SCCmec type IV(2B) | – | Unknown# | IS256, ISSau4, ISSep3 | 10 (0) | 0.944 (482) |
| C36 | MecA, blaZ, tet(M), aac(6′)-aph(2′′), aadD, cat(pC221), cat(pC233), erm(A), erm(C), norA | rep19b, repUS9, rep10, rep22, rep7a, rep7b, repUS43 | Type II | SCCmec type V(5C2) | – | ST54 | IS256 | 5 (0) | 0.942 (540) |
| C38 | MecA, blaZ, norA, erm(C) | rep10, repUS43 | Type II | SCCmec type IV(2B) | – | ST83 | ISSep3, ISSau4, IS256 | 3 (0) | 0.943 (555) |
| C40 | MecA, blaZ, aac(6′)-aph(2′′), erm(A), erm(C) | rep10, repUS9, repUS43 | – | – | – | ST54 | IS256 | 4 (0) | 0.944 (521) |
| C68 | MecA, blaZ, tet(M), tet(K), cat(pC221), erm(C), lsa(A), erm(B), dfrG | rep2, repUS11, rep10, rep7a, repUS43, repUS12 | – | SCCmec type XIII(9A) | III | ST210 | ISSau4, ISEfa11 | 6 (0) | 0.947 (501) |
| C81 | MecA, blaZ, norA, aac(6′)-aph(2′′), cat(pC221), erm(A), erm(C), erm(B) | rep7a, repUS43, rep2, repUS11, rep22, repUS46, repUS23, repUS9, rep10 | – | SCCmec type IV(2B) | – | ST2 | ISEfa11, ISSep3, ISSau4, IS256 | 5 (0) | 0.942 (531) |
| C119 | mecA, blaZ, erm(C), aac(6′)-aph(2′′), aac(6′)-Ic, dfrG | rep10, rep19b, rep19c, rep20, rep39, repUS70 | – | – | III | Unknown | IS16, ISSep2, IS256 | 6 (0) | 0.947 (171) |
| C122 | mecA, blaZ, aadD, aac(6′)-aph(2′′), mph(C), msr(A), dfrG | rep19b, rep19c, rep20, rep39, rep10, | – | SCCmec type I(1B) | I | ST59 | ISSep3, ISSau4 | 3 (0) | 0.951 (513) |
| C127 | mecA, blaZ, mph(C), msr(A), erm(C), dfrG | rep10, rep19b, rep19c, rep20, rep39 | – | SCCmec type I(1B) | I | ST59 | ISSau4, ISSep3 | 4 (0) | 0.949 (548) |
| C133 | blaZ | None | – | – | – | ST490 | ISSep3 | 4 (0) | 0.964 (152) |
| C135 | mecA, blaZ, norA, tet(K), msr(A), mph(C), cat(pC221), dfrG | rep7a, repUS70 | – | SCCmec type IVg(2B) | I | Unknown | ISSep3 | 3 (0) | 0.953 (394) |
| C137 | mecA, norA, blaZ, aac(6′)-aph(2′′), erm(A), erm(C), dfrG | rep10, repUS9, repUS43 | – | – | – | Unknown | IS256 | 1 (0) | 0.727 (36) |
| C138 | blaZ, dfrG | – | – | – | – | Unknown | ISSep3, ISSau4 | 7 (0) | 0.955 (328) |
| C145 | MecA, blaZ, tet(K), aac(6′)-aph(2′′), aph(3′)-III, erm(B) | rep11a, rep18b, rep2, rep7a | – | SCCmec type IVa(2B) | – | ST2 | ISSep3, ISSau4, ISEnfa3, IS16, ISEfa5, IS256 | 3 (0) | 0.968 (1) |
| C146 | MecA, blaZ, msr(A) | repUS48 | – | SCCmec type IVa(2B) | – | ST640 | IS30, ISEc36, ISSep2, ISSau4 | 4 (0) | 0.948 (121) |
| C148 | blaZ, tet(K) | rep21, rep19c, repUS9, rep5d, rep7a | – | – | – | Unknown | IS256 | 2 (0) | 0.948 (30) |
R-M system, restriction-modification system; ACME, arginine catabolic mobile element. ACME types I (arc and opp3 operons), II (the arc operon only), III (the opp3 operon only), IV (arc and kdp operons) and V (arc, opp and kdp operons). Pathogenicity score: prediction of a bacteria’s pathogenicity toward human hosts using PathogenFinder. Strain of the closet pathogenic family linkage: Staphylococcus epidermidis ATCC 12228. MRSE, methicillin-resistant Staphylococcus epidermidis; MLST, multilocus sequence typing; CRISPR-Cas, clustered regularly interspaced short palindromic repeats/CRISPR-associated. *SCCmec typing was predicted with the SCCmecFinder. #Unknown means a sequence type could not be assigned to isolate due to missing alleles in the draft genomes.