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Comparison of mitochondrial yield and cytoplasmic contamination between the Optimized and Kit methods. Total cell lysates, mitochondria, and cytoplasmic fractions were normalized by volume and resolved on 4–12% SDS PAGE and probed for Aconitase (mitochondrial matrix marker), TOMM 20 (mitochondrial outer membrane marker) and other organelle markers: PGK (cytoplasm), Cathepsin D (lysosome) and Histone H3 (nuclear).
