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. 2021 Jan 23;8:101238. doi: 10.1016/j.mex.2021.101238

Fig. 2.

Fig 2

Electropherograms showing results of quality check of libraries constructed from MiFish PCR amplicons. X and Y-axes represent fragment size (bp) and fluorescence intensity (relative fluorescence units, RFU), respectively. Left panels (YA2-3, YA2-4, YA2-5) were amplified using SuperFi II and right panels (YA1-16, YA1-5, YA1-7) were amplified using KAPA in the first PCR. Solid triangles indicate a peak representing MiFish-targeted amplicons. Open triangles indicate a peak representing nonspecific amplicons. LM: Lower marker. UM: Upper marker. YA2-3, YA2-4, YA2-5, YA1-5, YA1-7, and YA1-16 represent sample numbers.