Table 3.
Extracted eDNA yields from pumped deep-sea water collected at Akazawa using two different protocols. AK2-1, AK2-7, AK2-8, AK2-10, AK2-11, AK1-16, and AK1-17 represent sample numbers.
| Filter No. | Filtration volume (L) | Protocol | eDNA elution volume (µL) | Extracted eDNA concentration (ng/µL) | Total eDNA yield (ng) | eDNA yield per 1 L of filtered water (ng) |
|---|---|---|---|---|---|---|
| AK2-7 | 20 | modified | 200 | 13.0 | 2600 | 130.0 |
| AK2-8 | 20 | modified | 200 | 13.4 | 2680 | 134.0 |
| AK2-10 | 20 | modified | 200 | 14.4 | 2880 | 144.0 |
| AK2-11 | 20 | modified | 200 | 15.5 | 3100 | 155.0 |
| AK2-1 | 20 | conventional | 100 | 3.8 | 380 | 19.0 |
| AK1-16 | 30 | conventional | 100 | 6.8 | 680 | 22.7 |
| AK1-17 | 30 | conventional | 100 | 8.1 | 810 | 27.0 |