Table 3.
Composition of the regular LAMP reaction.
| LAMP component | Volume per reaction (µL) |
|---|---|
| Bst 2.0 (New England Biolabs) 8 U/µL | 1 |
| Isothermal Amplification Buffer (New England Biolabs) 10X | 2.5 |
| Betaine (Sigma Aldrich) 5 M | 4 |
| dNTPs (10 mM) | 3.5 |
| MgSO4 (100 mM) | 1.5 |
| Outer primers (F3 and B3; 1 µM) | 0.768 (each) |
| Inner primers (FIP and BIP; 10 µM) | 0.768 (each) |
| Loop primers (Loop F and Loop B; 10 µM) | 0.384 (each) |
| Nuclease-free water | 7.66 |
| Template (genomic DNA, heat-killed bacterial cells, seed extract or plant tissue exudate) | 1 |
| Total | 25 |
| Mineral oil | 20 |
| Quant-IT™ Pico GreenⓇ Reagent (Invitrogen, Carlsbad, CA, USA) | 0.5 |