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. 2021 Mar 26;8:101307. doi: 10.1016/j.mex.2021.101307

Table 3.

Mobile phases used in negative mode, with its gradient flow and time.

Negative ionization
Time (min) % Mobile Phase A* % Mobile Phase B^ Flow (mL/min)
0 95 5 0.3
3 50 50 0.3
6 10 90 0.3
13 0 100 0.3
17 0 100 0.3
18 95 5 0.3
20 95 5 0.3
⁎

A: H2O 5 mM AcNH4.

^

B: MeOH 5 mM AcNH4.