Fig. 2.

Automated workflow for STC analysis in KNIME. (a) Video files (AVI format) are converted to image stacks. (b) A threshold is applied to identify the location of the embryos in the image, (c) the binary image is segmented and (d) adjacent touching embryos are separated using morphological operations. (e) Variance of pixels between frames are identified to indicate movement. The graph shows the variance of one selected embryo. (f) Each peak (indicated by a dashed vertical line) represents an individual tail flip. Peaks were identified using an R script embedded in a KNIME workflow. The plot show the STC peaks of one embryo within a duration of 60 s or 120 frames.