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Establishing optimal coating concentration for the various peptides. Different coating concentrations (300 nM, 100 nM, 30 nM) of the various CSP C-terminal peptides were tested for reactivity with a human CSP-immune serum pool (1:3,000 dilution). The mean luminescence signal of the malaria-naïve serum pool (negative control) did not exceed the background (i.e., wells incubated with secondary antibody only (MLS < 1000 for all conditions)).
