Fig. 2.

Linear range of antibody response to peptides spans across four logs. Pooled human CSP-immune serum was tested at various dilutions for reactivity with the mutant peptides. The regression (red line) showed a fit with R² >0.97 for all peptides. Data are expressed as mean luminescence signal of two independent experiments for each panel (error bars represent standard deviation). Note different ranges on Y-axis depending on the reactivity of the antibodies to the respective peptide. % CV was less than 5% for all tests. Plots arranged in decreasing sieve Hamming Distance (left to right, top to bottom) from 3D7. The signal with malaria-naïve serum (negative control) did not exceed MSL<780 at the 1:1,000 dilution.