Fig. 3.

No evidence of antigenic competition. Plates were coated with (A) 300 nM (saturated concentration) or (B) 100 nM (limiting concentration) of peptides. Wells were either coated with only one specific peptide (single) or mixed as a cocktail (multiplex) and tested for reactivity with a human CSP-immune serum pool (1:3,000). The mean luminescence signal of the malaria-naïve serum pool (negative control) did not exceed the background (i.e., wells incubated with secondary antibody only (MLS < 1,000 for all conditions). Data expressed as mean luminescence signal (log-transformed).