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Sequence and parameters of primers for PCR.
| Gene names | Primer sequence (5′-3′) | Length (bp) |
|---|---|---|
| GAPDH | F: GAAGGCTGGGGCTCATCTG R: CAGTTGGTGGTGCACGATG |
150 |
| Occludin | F: AGATGGACAGCATCAACG R: CTGCCACATCCTGGTATT |
90 |
| Claudin-1 | F: CCTCCACAGGGAAGGATTAC R: ACCTAGAGTCTGAACTCTGC |
121 |
| Claudin-2 | F: CTCAGCCCTCCATCAAACA R: TGCTGCTGCTACACGTAT |
82 |
| ZO-1 | F: TGGTACTGACCAACGTAGTTC R: AGGAGTGACATCTAATAAAGCG |
94 |
| ZO-2 | F: ATGATAGCTGGTATGGTAGTCT R: CATGCGATCATCTGCGTC |
113 |
F, forward primer; R, reverse primer.
