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. 2021 Aug 5;12:719602. doi: 10.3389/fphar.2021.719602

FIGURE 1.

FIGURE 1

WEMA inhibits osteoclast differentiation. (A) MLO-Y4 cells and BMMs were co-cultured with or without WEMA (11.1, 33.3, 100, and 200 μg/ml) for 5 days in the presence of VitD3 (10 nM) or VitD3 (10 nM) plus RANKL (50 ng/ml). Representative images of TRAP staining (left panel; scale bar, 200 µm) and the number of TRAP-positive MNCs containing three or more nuclei (right panel). (B) BMMs were cultured with or without WEMA for 4 days in the presence of RANKL (50 ng/ml). Representative images of TRAP staining (upper panel; scale bar, 200 µm), TRAP activity (lower left panel), and the number of TRAP-positive MNCs (lower right panel) containing three or more nuclei. (C) BMMs were incubated with or without WEMA (11.1, 33.3, 100, and 200 μg/ml) for 24 h. Cell viability was evaluated by Cell Counting Kit-8 assay. Values are the mean ± SD of one representative experiment out of three with similar results, performed in triplicates. **p < 0.01 versus control.