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. 2021 Aug 5;12:719602. doi: 10.3389/fphar.2021.719602

FIGURE 2.

FIGURE 2

WEMA inhibits RANK-induced signaling required for NFATc1 induction. (A,B) BMMs were pre-treated with or without WEMA (200 μg/ml) for 3 h in the presence of M-CSF (60 μg/ml). Then, the cells were stimulated with RANKL (50 ng/ml) for 0, 1, 2, or 3 days. (A) The protein expression of AhR, c-Fos, and NFATc1 was analyzed by western blot. (B) The mRNA expression of c-Fos, NFATc1, Blimp1, IRF-8, MafB, cathepsin K, MMP-9, and integrin β3 was analyzed by qPCR. (C) BMMs were pre-treated with or without WEMA (200 μg/ml) for 3 h in the presence of M-CSF (60 μg/ml). Then the cells were stimulated with RANKL (50 ng/ml) for 0, 5, 15, or 30 min. The indicated proteins were analyzed by western blot. Values are the mean ± SD of one representative experiment out of three with similar results, performed in triplicates. *p < 0.05, **p < 0.01 versus control.