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(A) SDS-PAGE electrophoresis of COSE protein obtained by Sephadex G-75 chromatography. Lane M: standard molecular weight (kDa); Lane 1: 20 mg/ml protein; Lanes 2–3: 10 mg/ml protein. (B) Sephadex G-75 of COSE. (C) Analysis of COS hydrolyzates by HPLC. Blue peaks indicate mixed standard of COS degradation products. Black peaks represent COS degradation products by purified COSE from C4 strain.
