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. Author manuscript; available in PMC: 2021 Aug 19.
Published in final edited form as: Cancer Res. 2009 Apr 7;69(8):3570–3578. doi: 10.1158/0008-5472.CAN-08-2807

Figure 3.

Figure 3.

Chromatin immunoprecipitation analysis of histone alterations coinciding with Dkk-1 expression in cultured lung cancer cells. A, chromatin immunoprecipitation analysis of the Dkk-1 promoter in A549 and Calu-6 cells cultured in NM ± TSC (0.003 puff/mL) for 10 d. SW480 and DLD1 colon cancer cells were used as controls for activation and repression marks, respectively. B, chromatin immunoprecipitation analysis of polycomb proteins recruited to the Dkk-1 promoter in cultured lung cancer cells following TSC exposure. C and D, chromatin immunoprecipitation analysis of the Dkk-1 promoter (C) and quantitative RT-PCR assessment of Dkk-1 expression in Calu-6 cells (D) before and after removal of TSC from culture media.