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. Author manuscript; available in PMC: 2021 Aug 19.
Published in final edited form as: Cancer Res. 2009 Apr 7;69(8):3570–3578. doi: 10.1158/0008-5472.CAN-08-2807

Figure 4.

Figure 4.

Effects of EZH2 and SirT1 knockdown on TSC-mediated repression of Dkk-1 in cultured lung cancer cells. A, Western blot analysis of EZH2 or SirT1 protein levels in Calu-6 lung cancer cells following TSC exposure. B, Western blot analysis of EZH2 and SirT1 protein levels in Calu-6 cells transfected with siRNAs against EZH2 or SirT1 or with sham control sequences showing specific target gene knockdown. C, quantitative RT-PCR analysis of Dkk-1 expression in Calu-6 cells exhibiting siRNA-mediated knockdown of EZH2 and/or SirT1.D, Western blot analysis of Dkk-1 expression in untreated and TSC-exposed Calu-6 cells transfected with sham siRNAs or siRNAs targeting EZH2 and/or SirT1. Corresponding densitometry showing that knockdown of EZH2 and, to a lesser extent, SirT1 increases Dkk-1 protein levels in untreated Calu-6 cells and partially abrogates the depletion of Dkk-1 mediated by TSC.