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. 2021 Jul 26;24(8):102905. doi: 10.1016/j.isci.2021.102905

Figure 3.

Figure 3

Dispase improves antibody penetration for immunolabeling of intact eyes

Optical sections of pigmented (A, B, E, F) Ccdc-GFP or (C, D, G, H) C57BL/6J retinas immunostained with SYTOX Orange nuclear stain and anti-GFP or anti-Brn3a (A, B, C, D) without and (E, F, G, H) with dispase treatment. High background staining at the sclera is present between the dashed lines in B’. Arrows in D′ indicate high background staining at the sclera. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; OS, outer segment. Scale bars = 500 μm for A, C, E, G and 100 μm for B, D, F, and H.