Table 3.
PCR genotyping primers for respective mouse lines.
| Genotype | Target | Forward primer | Reverse primer |
|---|---|---|---|
| Pigmented C57BL/6J | NA | NA | NA |
| Pigmented Ccdc-136GFP/GFP | Wild type | CCGTGGTGGGGGTTGAATCCAA | TGGCAAAGTCATGAAGGGACCACA |
| Pigmented Ccdc-136GFP/GFP | Mutant | CACATGAAGCAGCACGACTT | TGCTCAGGTAGTGGTTGTCG |
| Pigmented Rbloxp/loxP; p107−/−; α-Cre | Rb floxed | CGC CGC ATA ACC AGT GAA AC | CTC AAG AGC TCA GAC TCA TGG |
| Pigmented Rbloxp/loxP; p107−/−; α-Cre | p107−/− | TCG TGA GCG GAT AGA AAG | GTG TCC AGC AGA AGT TA |
| Pigmented Rbloxp/loxP; p107−/−; α-Cre | Cre | ATG TCC AAT TTA CTG ACCG | CGC CGC ATA ACC AGT GAA AC |
| Pigmented Rbloxp/loxP; p107−/−; p27+/−; α-Cre{ | p27+/+ | GCC TGG CTC TGC TCC ATT TGA C | CTC TCC ACC TCC TGC CAT TC |
| Pigmented Rbloxp/loxP; p107−/−; p27+/−; α-Cre | p27−/− | CCT TCT ATG GCC TTC TTG ACG | TGG AAC CCT GTG CCA TCT CTA T |
| Pigmented Ndp−/Y | Wild Type | NA | CAGGGAGAGCATAGAAATGG |
| Pigmented Ndp−/Y | Mutant | TCTGATTTCATTCCAGCTGTG | CCCTAGGAATGCTCGTCAAGA |
| Nrl-GFP | Wild type | GTGTTCCTTGGCTGGAAAGA | CTGTTCACTGTGGGCTTTCA |
| Nrl-GFP | Mutant | CACATGAAGCAGCACGACTT | TGCTCAGGTAGTGGTTGTCG |
| Albino ROSAmT/mG | Wild type | GGA GCG GGA GAA ATG GAT ATG | NA |
| Albino ROSAmT/mG | Mutant | GCG AAG AGT TTG TCC TCA ACC | NA |
| Albino ROSAmT/mG | Common | NA | AAA GTC GCT CTG AGT TGT TAT |
| Albino Nrl−/−;ROSAmT/mG | Nrl wild type | GTGTTCCTTGGCTGGAAAGA | CTGTTCACTGTGGGCTTTCA |
| Albino Nrl−/−;ROSAmT/mG | Nrl mutant | TGAATACAGGGACGACACCA | GTTCTAATTCCATCAGAAGCTGAC |
| Albino Nrl−/−;ROSAmT/mG | ROSA wild type | GGA GCG GGA GAA ATG GAT ATG | NA |
| Albino Nrl−/−;ROSAmT/mG | ROSA mutant | GCG AAG AGT TTG TCC TCA ACC | NA |
| Albino Nrl−/−;ROSAmT/mG | ROSA Common | NA | AAA GTC GCT CTG AGT TGT TAT |