Figure 1.

Rat spinal cord injury model establishment.

(A) Spinal cord morphology in sham and spinal cord injury groups assessed by hematoxylin and eosin staining. Spinal cord sections of sham animals appeared smooth, whereas many cracks were apparent in those of spinal cord injury model animals (arrow). (B) The general morphology of the spinal cord. The spinal cord tissue of sham animals appeared smooth, whereas that of spinal cord injury model animals appeared uneven and hyperemic (arrow). (C, D) MBP (C) and GFAP (D) (red, Alexa Fluor 555) immunopositivities in spinal cord tissues. DAPI (blue) was used to stain nuclei. Sham rat sections appeared smooth, whereas sections from SCI model rats exhibited evidence of edema, inflammatory cell infiltration, and consolidation of the spinal cord nucleus. Scale bars: 500 μm in A, 5 mm in B, 200 μm in C and D. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; MBP: myelin basic protein.