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. 2021 Apr 23;16(12):2446–2452. doi: 10.4103/1673-5374.313056

Figure 2.

Figure 2

Immunofluorescence staining of cultured mouse and monkey cortical neurons at different time points.

(A) Double immunofluorescence staining of cultured mouse and monkey cortical neurons, using antibodies against GFAP and β-tubulin III, on days 3, 7, 11, and 14 (left column: magnification 10×, scale bars: 200 μm; middle and right columns: magnification 40×, scale bars: 20 μm; taken using a Zeiss Axio Observer A1 microscope system). The blue color indicates DAPI. (B) Double immunofluorescence staining of cultured monkey cortical neurons, using antibodies against GFAP and β-tubulin III, on day 81 (taken using a Zeiss LSM880 microscope system). Scale bar: 200 μm. DAPI: 4′,6-Diamidino-2-phenylindole; Gfap: glial fibrillary acidic protein.