Fig. 4.

Deletion of the CAP64 gene affects pigmentation in cryptococcal cultures. Pigmentation of C. neoformans strains was observed after incubating in asparagine medium for 24 h (a). The laccase activity of each strain was quantified by measuring the absorbance of pigments contained in the culture supernatant (b), and the transcription level of LAC1, which encodes laccase, was examined for each strain after culturing in asparagine medium for 3 or 6 h (c). Student’s t-test was used to compare them. Experiments were carried out in triplicate. Values are reported as the mean±SD. *Statistically significant by Student’s t-test (P<0.05).